Leica DMI4000B Microscope & Magnifier User Manual


 
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8. Operation
IP0 for 5x, e.g. NPlan 5x 11506087 objective with eyepoint A
IP1 for 10x, e.g. NPlan 10 x 11506084 objective with eyepoint A and
for 20x, e.g. NPlan L 20 x 11506200 objective with eyepoint C
IP2 for 40x, e.g. HCX PL FL L 40 x 11506201 objective with eyepoint C
IP3 for 63x, e.g. PL FL 63x/0.70 11506216 objective with eyepoint C
8.2.6 Integrated Phase Contrast (TL)
Leica DMI3000 B:
Select a bright field objective with eyepoint A
or C.
Select the appropriate light ring at the con-
denser (see table).
Open the aperture of the condenser com-
pletely.
Remove all other optical components such as
analyzers, polarizers or IC prisms from the
beam path.
Slide the phase contrast front module to the
correct eyepoint, A or C.
Insert a phase contrast specimen.
Set the brightness at the light potentiometer
Focus the image with the focus wheels.
Leica DMI4000 B and Leica DMI6000 B:
Use the TL/IL function button to switch to
transmitted light (TL).
Select the IPC contrast method (integrated
phase contrast). by pressing the variable but-
ton IPH. Alternatively: press the variable but-
ton CHANGE TL . (For details on button as-
signments, please see the identification
sheet.)
PH will appear on the LeicaScreen. Motorized
condensers will now switch to the correct
light ring. Coded condensers must be
switched manually.
Insert a transmitted light specimen.
Select a suitable objective (eyepoint A or C).
Slide the phase contrast front module to the
correct eyepoint, A or C.
Focus the image with the knob on the
SmartMove or the focusing wheel and adjust
the intensity with the INT function buttons.
Note:
When selecting the phase contrast method, the
aperture diaphragm is opened fully and can not
be adjusted.