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8. Operation
8.2.2 Phase Contrast (TL)
(integrated phase contrast, see 8.2.6)
Leica DMI3000B:
• Select a phase contrast objective.
• Select the suitable light ring on the con-
denser.
• Open the aperture of the condenser com-
pletely.
• Remove all other optical components such as
analyzers, polarizers or IC prisms from the
beam path.
• Insert a phase contrast specimen.
• Set the brightness at the light potentiometer
• Focus the image with the focus wheels.
Leica DMI4000B and Leica DMI6000B:
• Use the TL/IL function button to switch to
transmitted light (TL).
• Select the PH (phase contrast) contrast
method
by pressing the variable button PH.
Alternatively: press the variable button
CHANGE TL .
(For details on button assignments, please see
the identification sheet.)
PH will appear on the LeicaScreen.
Motorized condensers will now switch to the
correct light ring. Coded condensers must be
switched manually.
• Insert a transmitted light specimen.
• Rotate an appropriate objective into place.
Objectives that are suitable for phase contrast
are engraved with PH.
• Focus the image with the knob on the
SmartMove or the focusing wheel and adjust
the intensity with the INT function buttons.
Note:
When selecting the phase contrast method, the
aperture diaphragm is opened fully and can not
be adjusted.