Leica DMI4000B Microscope & Magnifier User Manual


 
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8. Operation
8.2.3 Dark Field (TL)
Note:
The maximum usable objective aperture for dark
field is for the condenser S1 0.70 and for the
condenser S23/S28 0.40.
Leica DMI3000B:
Select a dark field objective.
Select the suitable dark field stop on the con-
denser.
Open the aperture of the condenser com-
pletely.
Remove all other optical components such as
analyzers, polarizers or IC prisms from the
beam path.
Insert a dark field specimen.
Set the brightness at the light potentiometer
Focus the image with the focus wheels.
Leica DMI4000B and Leica DMI6000B:
Use the TL/IL function button to switch to
transmitted light (TL).
Select the DF (dark field) contrast method
by pressing the variable button BF.
Alternatively: press the variable button
CHANGE TL .
(For details on button assignments, please see
the identification sheet.)
DF will appear on the LeicaScreen.
Motorized condensers will now switch to the
dark field ring. Coded condensers must be
switched manually.
Insert a transmitted light specimen.
Rotate an appropriate objective into place.
Focus the image with the knob on the
SmartMove or the focusing wheel and adjust
the intensity with the INT function buttons.
When selecting the dark field method, the aper-
ture diaphragm is opened fully and can not be
adjusted.