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7. Start-up
Integrated phase contrast with bright field
objectives via front slider
When choosing an objective suitable for phase
contrast, the appropriate light ring is selected
automatically when using a motorized con-
denser. Otherwise, select the light ring manually.
Centering the phase rings is not required for ob-
jectives with eyepoint A. Checking the position
of the phase rings is essential only when using
objectives with eyepoint C.
(For the eyepoint of your objective, please refer
to the included objective list or the engraving on
the objective itself.)
• Move the front slider with the phase rings into
the beam path.
Leica DMI3000B:
• Set the condenser to the bright field position.
Leica DMI4000B and Leica DMI6000B:
• Press the BF (bright field) button (one of the
variable function buttons on the stand).
• Select the objective with the lowest magnifi-
cation.
• Focus on the specimen.
• Select the objective with the lowest magnifi-
cation and eyepoint C.
Leica DMI3000B:
• Select the light ring for your current objective
on the condenser.
Leica DMI4000B and Leica DMI6000B:
• Press the PH (phase contrast) button (one of
the variable function buttons behind the focus
wheels). The light ring will be selected in the
condenser.
• Slide the front slider with the phase rings to
position C (A and C refer to the eyepoint of the
objective. For the eyepoint of your objective,
please refer to the included objective list or
the engraving on the objective itself.)
• Instead of an eyepiece, place a focusing tel-
escope (Fig. 93) in the observation tube or ac-
tivate the Bertrand lens (pull rod (94.1) on
tube).
• Focus the ring structure (95a) by loosening the
clamping ring (93.2) somewhat and moving the
eyelens (93.1), or focus the Bertrand lens
(94.2).
• Retighten the clamping ring.
• If the light ring and the phase ring are not
shown as arranged in Fig. 95c, the light ring
must be centered.
• Insert the centering key in the opening pro-
vided on the front slider
•Turn the centering keys until the dark ring
(phase ring in the objective) is congruent with
the slightly narrower bright ring (light ring in
condenser) (95 c).
• Remove the centering keys after centering.