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8. Operation
8.2.4 Polarization (TL)
Leica DMI3000B:
• Select an objective.
• Set the condenser to the bright field position.
Remove all IC prisms from the light path.
• Move the polarizer on the condenser into the
beam path.
• Insert the analyzer into the right side of the
stand until it clicks into position.
• Bring the polarizer and analyzer into cross po-
sition until they reach maximum darkness.
• Insert a specimen.
• Set the brightness at the light potentiometer
• Focus the image with the focus wheels.
Leica DMI4000 B and Leica DMI6000 B:
• Use the TL/IL function button to switch to
transmitted light (TL).
• Select the POL (polarization) contrast method
by pressing the variable button POL.
Alternatively: press the variable button
CHANGE TL .
(For details on button assignments, please see
the identification sheet.)
POL will appear on the LeicaScreen.
Manual method:
• Move the polarizer on the condenser into the
beam path.
• Insert the analyzer into the right side of the
stand until it clicks into position (Fig. 110).
• Bring the polarizer and analyzer into cross po-
sition until they reach maximum darkness.
• Place a specimen on the stage and select a
suitable objective.
Motorized method:
• If the microscope is equipped with the rel-
evant components, the polarizer will be acti-
vated automatically in the condenser when
the POL contrast method is selected. The
analyzer cube is also automatically positioned
in the beam path.
Combined methods:
• The Leica DMI4000 B and Leica DMI6000B mi-
croscope permit purely mechanical and mo-
torized components – such as a mechanical
analyzer and motorized polarizer – to be com-
bined.
Fig. 110 Inserting the analyzer