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8. Operation
8.2.5 Differential Interference Contrast (TL)
Leica DMI3000B:
• Select an objective.
• At the condenser, select the appropriate
Wollaston prism condenser.
• At the objective turret, select the appropriate
Wollaston prism objective.
• Move the polarizer on the condenser into the
beam path.
• Insert the analyzer into the right side of the
stand until it clicks into position.
• Insert a specimen.
• Set the brightness at the light potentiometer
• Focus the image with the focus wheels.
• Use the knurled wheel below the objective
turret for fine adjustment (Fig. 111).
Leica DMI4000B and Leica DMI6000 B:
• Use the TL/IL function button to switch to
transmitted light (TL).
• Select the DIC contrast method
by pressing the variable button DIC.
Alternatively: press the variable button
CHANGE TL .
(For details on button assignments, please see
the identification sheet.)
DIC will appear on the LeicaScreen.
• The polarizer in the condenser and the suit-
able condenser prism are automatically posi-
tioned in the beam path. The corresponding
objective prism and the analyzer cube are
also positioned automatically.
• Place a DIC specimen on the stage.
• Rotate an appropriate objective into place.
• Focus the image with the knob on the
SmartMove or the focusing wheel and adjust
the intensity with the INT function buttons.
• Use the knurled wheel below the objective
turret for fine adjustment (Fig. 111).
Manual alternative:
• Move the polarizer on the condenser into the
beam path manually.
• Insert the analyzer manually into the right side of
the stand until it clicks into position (Fig. 110).
Adjust the objective and condenser prisms
manually until a valid combination appears on
the display.
• Use the knurled wheel below the objective
turret for fine adjustment (Fig. 111).
Fig. 111 DIC disk with knurled wheel for fine adjustment