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Operation of LMC
Leica modulation contrast (LMC) is a special
form of oblique illumination based on the
principle of Hoffmann modulation contrast.
In this technique, the phase gradients of an
unstained specimen are converted into dif-
ferences in amplitude with the aid of a modu-
lator.
This gives a three-dimensional impression simi-
lar to an interference contrast image. Unlike
interference contrast, however, the specimen
can be observed through birefringent plastic
materials such as petri dishes.
Further advantages of this technique are:
– high contrast
– high resolution
– halo-free, high-contrast relief image
– long free working distance of the condenser
– easy assembly and adjustment
–
use for both stained and unstained specimens.