Leica DM IRB Microscope & Magnifier User Manual


 
81
The BG 38 filter should always be used for
photography.
When not looking through the microscope,
always block the incident light path to prevent
specimens fading. Push the switch rod in all the
way.
The 3 clickstop positions of the switch rod mean:
Switch rod
Stop Incident light path blocked
(light stop)
BG 38 BG filter engaged
Incident light path open
n. b.:
Only with microscope with integrated incident
light fluorescence axis.
Fluorescence observation
Focus the specimen in transmitted light first, if
possible (perhaps Phaco or ICT).
Select a filter cube to suit the excitation and
emission spectrum of the specimen and move
into the light path by rotating the turret. Open the
iris diaphragm of the objective.
Switch the magnification changer* (on the
DM IRB-SLR and DMIRBE versions) by turning
the knurl to pos. 1x. Switch off the transmitted
light illumination.
Open the incident light path. The switch rod
should be pulled out fully.
If the background is too red, engage a BG 38
filter. Push the switch rod in halfway.
Operation of incident light fluorescence