Leica DM IRB Microscope & Magnifier User Manual


 
77
DL polarisation
Polarisation contrast for examining birefringent
specimens is possible with condenser 0.30 S70
with objective magnifications from 2.5x to 40x,
with condensers 0.53 S23 or 0.90 S1 from 5x or
10x to 100x. A P 1.40 OIL S1 condenser top is also
available for extremely hgh resolution.
Crossing the polarisers
First: Set Koehler illumination. Remove the speci-
men from the light path; remove the Bertrand
lens and fluorescence filter cube if necessary;
turn the condenser disc and turret for objective-
side IC prisms to pos. H.
Insert the polariser into the filter holder with the
engraving facing upwards. Turn the filter holder
to the right into the light path.
Push the analyser into the 2nd clickstop position
in the microscope with the engraving ICT facing
upwards.
Set the optimum extinction position by rotating
the polariser and watching the empty field of
view. Put a specimen on the stage.
For Pol colour contrast, the ICT analyser can be
turned over, with the lambda engraving facing
upwards, to activate a whole-wave compensator.
Operation of transmitted light polarisation