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Length measurements
The following components are required for
length measurements:
– Graticule with scale in eyepiece (Fig. 76) or in
the slide overlay device (Fig. 74).
– Transmitted light stage micrometer for cali-
bration.
Before measurement, the
micrometer value of
the objective/eyepiece combination must be
known, i. e. the distance in the specimen that
corresponds to a scale interval in the graticule
you are using.
Calibration:
Align the stage micrometer and the graticule
parallel to one another by rotating the eyepiece
and adjust the zero marks of the two scales to
exactly the same height (Fig. 76).
Read how many scale divisions of the stage
micrometer correspond to how many on the
microscope scale (graticule) and divide the two
values.
Example:
If 1.220 mm of the stage micrometer corre-
sponds to 100 divisions of the measurement
scale, the micrometer value is = 1.220 :100 =
0.0122 mm = 12.2 µm. For extremely low
objective magnifications it may be that only part
of the measurement scale can be used for
calibration.
Important: If using a magnification changer*
(DM IRB-SLR and DMIRB/E stands):
Remember to take the additional magnification
value into consideration separately instead of
extrapolating the micrometer values of the other
objectives from the calibration of one objective.
Measurement errors may occur if the eyepiece
is not pushed into the tube as far as the stop.
Connections for TV cameras and
photomicro equipment
All the variants of the Leica DM IRB stand have
a photo/TV exit on the left side.
There are also photo/TV exits in the trinocular
tubes for vertical adaption of camera systems.
Fig. 76 Graticule scale in the eyepiece (left) and image of the
stage micrometer (right)