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11. Troubleshooting
Problem
Polarization
No polarization contrast is possible.
The image is insufficient.
The image is too dark.
No conoscopy image is possible.
Fluorescence
The image is completely dark (no fluorescence).
The fluorescence is too weak.
Display
The display flashes.
FAIL! appears on the display.
Cause/remedy
Bring the polarizer and analyzer into cross po-
sition until they reach maximum darkness
(without specimen).(→ p. 61ff, 72).
The embedding material for transmitted light
specimen is birefringent.
Polarizers can be damaged by strong light
sources. Check to see if the polarizer is dam-
aged (discolored) or heavily soiled.
Check to see if a beam splitter or filter is
switched on.
Objectives and condensers can be deformed
by mechanical damage.
Open the shutter (→
p. 70).
Select the incident light axis (IL) (→
p. 40).
Check the antigen-antibody combination.
Insert the booster (→
p. 33).
Center the lamp (→
p. 47ff).
Insert a new lamp (→
p. 23f).
Iotate an appropriate objective for the con-
trast method into place.
Check the installed objectives, filter cubes, etc.
Switch the microscope off and back on.