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10. Contrast methods for Leica DM4000 M
10.1.4 Interference contrast (RL)
• Switch to the incident light axis (IL) by push-
ing the TL/IL function key.
• Insert a specimen and rotate a suitable objec-
tive into place.
• Select the DIC contrast method
Do so by pressing the DIC variable key.
Alternatively: press the variable button
CHANGE RL
.
(For details on key assignments, please see
the identification sheet.)
The display indicates ICR.
• The ICR filter cube (containing the polarizer
and analyzer) is automatically brought into the
beam path on the incident light axis. Insert the
objective prism slide into the prism slot
(Fig. 77.2).
Alternatively:
• Manually push the ICR polarizer (77.3) and the
IC/P analyzer (78.1) on the stand into the beam
path.
• Then insert the objective prism slider into the
slot on the objective turret. (77.2).
• For fine adjustment, rotate the knurled screw
(77.1) on the objective prism slide.
10.2 Transmitted light
10.2.1 Bright field (TL)
• Switch to the transmitted light axis (TL) by
pushing the TL/IL function key.
• Select the BF (bright field) contrast method
Do so by pressing the BF TL variable key.
(For details on key assignments, please see
the identification sheet.)
The display indicates BF TL.
• Insert a transmitted light specimen.
• Rotate an appropriate objective into place.
• Bring the image into focus using the focus
wheel and set the brightness using the INT
function key.
10.2.2 Polarization (TL)
• Switch to the transmitted light axis (TL) by
pushing the TL/IL function key.
• Select the POL (polarization) contrast method.
• Push the analyzer on the left side of the stand
into the beam path (Fig. 78).
• Turn the polarizer on the underside of the con-
denser into the light path. Make sure that the
red index point on the front of the polarizer is
at 0 (Fig. 72).