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41
7. Startup
Fig. 40 Condenser centering
1 Centering screws
11
7.5 Köhler illumination
7.5.1 Transmitted light
Suitable aperture and field diaphragm values
have been preset for each objective. The con-
denser is also already centered at the factory.
However, depending on how the condenser is
disassembled and reassembled, it may be nec-
essary to re-center the condenser in some cas-
es. Therefore, check the condenser centering.
The following procedure is provided for the
transmitted light-bright field illumination.
• Select an objective with moderate magnifica-
tion (10x-20x).
• Push the TL/IL button (38.1) as needed to acti-
vate the transmitted light axis. "TL" appears in
the first line of the display.
Note on DM4500 P: Ensure that the Bertrand
lens is swiveled outwards.
Fig. 39 Stage with specimen holder
1 Object motion (X direction)
2 Object motion (Y direction)
3 Specimen holder
4 Condenser height adjuster
• Press the BF button to activate the bright field
contrast method (one of the variable function
keys behind the focus wheel).
"TL BF" appears in the first line of the display.
• Insert the specimen in the stage’s specimen
holder (39.3).
• Focus on the specimen. The focus wheel on
the left side of the stand allows focus adjust-
ment in course and fine increments. On the
right side of the stand, there is also a focus
wheel for fine focusing.
• Adjust the light intensity with the INT buttons
(38.3).
• Close the field diaphragm with the FD function
key (38.2) until the edge of the diaphragm ap-
pears in the specimen plane.
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