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42
7. Startup
• Using the condenser height adjuster (39.4),
adjust the condenser until the edge of the
field diaphragm appears in sharp relief.
• If the image does not appear in the middle of
the field of view (41c), the condenser must be
moved into the middle of the field of view with
the help of the two centering screws (40.1).
• Open the field diaphragm just enough for it to
disappear from the field of view (41d).
Caution:
The condenser height adjustment depends on
the thickness of the specimen. It may need to be
adjusted for each specimen.
a
b
cd
Fig. 41 Köhler illumination
a Field diaphragm not focused, not centered
b Field diaphragm focused, but not centered
c Field diaphragm focused and centered
Diameter is too small, however
d Field diameter (light) = Field diameter (view)
(Köhler illumination)
7.5.2 Incident light
Suitable aperture and field diaphragm values
have been preset for each objective. The inci-
dent light module has also been centered at the
factory.
However, it may be necessary to readjust the
incident light module in some cases after trans-
porting and setting up the stand. Therefore,
check the aperture and field diaphragm center-
ing.
The following procedure is provided for the inci-
dent light-bright field illumination.
• Select an objective with moderate magnifica-
tion (10x-20x).
• Activate the incident-light axis with the TL/IL
button (38.1).
"IL" appears in the first line of the display.
• Select the bright field contrast method by
pressing the IL-BF button (DM4000 M) or se-
lect fluorescence by pressing the FLUO button
(DM4000 B, DM5000 B).
These functions can be assigned to the vari-
able function keys on the stand.
IL BF / FLUO appears in the first line of the dis-
play.
• Insert the specimen in the stage’s specimen
holder (39.3).
• Focus on the specimen with the focus wheels.
• Adjust the light intensity using the INT buttons
(38.3).