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9. Contrast methods for Leica DM4000 B/DM4500 P/DM5000 B
9.1.3 Dark field (TL)
• Switch to the transmitted light axis (TL) by
pushing the TL/IL function key.
• Select the DF (dark field) contrast method by
pressing the variable button DF.
Alternatively: press the variable button
CHANGE TL
.
(For details on key assignments, please see
the identification sheet.)
The display indicates DF.
The dark field ring (dark field stop) is set auto-
matically in the condenser.
• Insert a transmitted light specimen.
• Rotate an appropriate objective into place.
• Bring the image into focus using the focus
wheel and set the brightness using the INT
function key.
Notes:
The maximum objective aperture that can be
used for dark field is 0.75. All objectives with
greater apertures are automatically blocked for
this method ("DF" flashes in the display).
The microscope automatically selects the cor-
rect light ring in the condenser.
When the dark field method is selected, the ap-
erture diaphragm is opened fully and cannot be
adjusted.
9.1.4 Polarization (TL)
• Switch to the transmitted light axis (TL) by
pushing the TL/IL function key.
• Select the POL (polarization) contrast method
by pressing the variable button POL.
Alternatively: press the variable button
CHANGE TL
.
(For details on key assignments, please see
the identification sheet.)
The display indicates POL.
9.1.4.1 Manual method
• Rotate the polarizer on the underside of the
condenser into the beam path (Fig. 67a). Make
sure that the red index point on the front of
the polarizer is at 0.
• Insert the analyzer up to the stop into the left
side of the stand (Fig. 67b.1).
• Bring the polarizer and analyzer into cross po-
sition until they reach maximum darkness.
• Insert a specimen and rotate a suitable objec-
tive into place.