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11. Trouble shooting
Problem
Phase Contrast
No phase contrast is possible.
Fluorescence
The image is completely dark (no fluorescence).
The fluorescence is too weak.
Stage
Travel range of the stage in e-w direction
decreases after a longer period of working.
Cause/Remedy
The specimen is too thick, too thin or too
brightly stained
Refractive index of mounting medium and
specimen is identical so that there is no phase
jump
The cover glass is not placed evenly
Check the right light ring (→ p. 44)
Check the centering of the light rings
(→ p. 29-30)
Check the condenser centering
Open the aperture diaphragm completely
Open the shutter (→
p. 46)
Check the antigen-antibody combination
Insert a new lamp (→
p. 21 onwards)
Center the lamp (→
p. 31 onwards)
Insert a new lamp (→
p. 21 onwards)
Move the stage all the way to the left side
with the coaxial pinion
Push one of the screws which hold the object
guide with your hand further to the left as far
as it will go
Afterwards move the stage all the way to the
right
Push one of the screws which hold the object
guide with your hand further to the right as far
as it will go