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30
7. Start-up
Fig. 28 Light ring centering (i.e.: condenser UCL/P)
1 Centering keys
11
• In the place of an eyepiece, insert the focus-
ing telescope (fig. 27) into the observation
tube
• Swivel in the phase contrast objective with
the lowest magnification
• Focus on the specimen with the focus wheel
• Focus the ring structure (29.a) by slightly loos-
ening the clamping ring (27.2) and moving the
eye lens (27.1)
• Retighten the clamping ring
• Select the corresponding ring diaphragm
(light ring) in the condenser.
• If the light ring and the phase ring are not
shown as arranged in fig. 29.c, the light ring
must be centered
• Insert the centering screws into the openings
provided at the rear of the condenser (28.1)
• Turn the centering screws until the dark ring
(phase ring in the objective) is congruent with
the slightly narrower bright ring (light ring in
condenser) (29 c)
• Repeat the process for all other light rings and
objectives
• Optionally remove the centering keys after the
centering procedure
Fig. 27 Focusing telescope
1 Adjustable eye lens
2 Clamping ring for fixing the focus position
1
2
PH LR
Fig. 29 Phase contrast centering procedure
PH=phase contrast ring, LR=light ring
a Condenser in bright field (BF) position
b Condenser in phase contrast (PH) position
Light ring (LR) not centered
c Light ring and phase ring centered
ab c