Leica DM1000 LED Microscope & Magnifier User Manual


 
52
11. Trouble shooting
Problem
Fluorescence: The lamp does not illuminate
immediately upon being switched on.
Focus
The specimen cannot be brought into focus.
Darkfield
No definite DF contrast is possible.
The image is unevenly or not uniformly
illuminated.
Undesirable stray light.
Polarization
No polarization contrast is possible.
Cause/Remedy
The external power supply must be switched
on repeatedly
Hot Hg lamps should cool down before
switching on again
Use the correct immersion medium
Lay the specimen with the cover glass to-
wards the top
Make sure that the cover glass thickness is
correct and that it suits the indication on the
objective
Be sure that a DF objective is being used
The objective aperture setting is too high
(maximum 0.75/1.10); if necessary, reduce the
objective aperture using the iris diaphragm on
the objective
Check the condenser centering
Open the aperture diaphragm completely
The magnification is too weak. Use a higher
magnification
Clean the specimen and neighboring lenses
(
p. 54)
Bring the polarizer and analyzer into cross po-
sition until they reach maximum darkness
(without specimen) ( p. 45)