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45
9. Contrast Methods
9.1.4 Oblique Illumination*
• First adjust transmitted light darkfield
• To obtain a relief-like contrast:
Condenser UCA/P:
Rotate condenser disk slightly out of the DF
position
Condensers CL/PH, CLP/PH, and
APL. ACHR.0.9 (P):
Push DF slide in part way out of the DF
position
9.1.5 Polarization*
• Swing the lambda plate of the lambda plate
compensator out if your microscope is
equipped with it
• Insert a specimen and rotate an appropriate
objective into place
• Bring the image into focus and set the Köhler
illumination (→
p. 28)
• Depending on the equipment, the analyzer
may already have been inserted into the tube
mount during the assembly
Alternative:
If the analyzer mount TL* is used:
Insert the analyzer as far as the clickstop into
the microscope stand. The engraving λ must
be on the underside
When using the Pol intermediate tube*:
Switch on the analyzer
• Push the polarizer with the labelled side
upward into the lower opening
Attention!
Always use the polarizer with the labelled
side upward, as otherwise the integrated heat
protection filter is ineffective and the special
polarizer will become useless (discoloring!).
• Bring the polarizer and analyzer into cross po-
sition until they reach maximum darkness
• Remove the object or find an empty area of
the specimen
• Push the analyzer into the stand as far as
the 2nd clickstop or switch on the module
• Remove compensators from the light path if
your microscope is equipped with it
• Rotate the polarizer until you observe the
maximum extinction position in the
eyepiece (fig 48)
• Fix the cross position thus determined with
the clamping screw
!
Fig. 48
Crossing the polarizers when observing through a focusing
telescope or Bertrand lens, high-aperture Pol objective
a exactly crossed, b not exactly crossed
Pos. a cannot be set if there is strain in the condenser or
objective, Pos. b is adequate for polarization contrast.
a
b