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43
9. Contrast Methods
9.1.1 Brightfield
• If present: click the condenser disk* into the
BF position
• If present: pull the light ring slide* out of the
condenser
• If present: switch the fluorescence illuminator
into an empty position or filter system A
• Insert a transmitted light specimen
• Rotate an appropriate objective into place
• Movable condenser heads:
The condenser top is swung out for
objective magnifications < 10x
• Bring the image into focus using the focus dial
and set the brightness
• For an optimal field diaphragm setting, check
the Köhler illumination (→
p. 31)
• Use suitable transmitted-light filters as
applicable (fig. 47)
Fig. 47 Filter holders*
DLF filter magazine for
attachment to micro-
scope base
Filter holder for screw
attachment on the under-
side of the condenser
Filter holder with
two positions or one po-
sition for attachment to
microscope base