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Leica M-series User Manual 121
Observation without Fluorescence
We recommend that you first familiarize
yourself with the controls of your Leica
fluorescence microscope in transmitted light
without the presence of fluorescence illumina-
tion.
•
After switching on the high-pressure
mercury burner, you must adjust the arc
lamp immediately (see User Manual M2-216-1).
We recommend that you first familiarize
yourself with the basic functions of your
Leica stereomicroscope as they are described in
the previous section of this User Manual. These
include the binocular tube, the eyepieces, the
zoom magnification changer, the focusing
drive, and others.
The following describes the specific functions
of the fluorescence system only.
Observation without Fluorescence
The FLUOIII® filter system includes a UV shutter
that can be used to close the illumination beam
path. This feature protects the mercury lamp,
which should not be switched on and off too
frequently. If, for instance, you need to inter-
rupt your work, you do not have to switch off
the high-pressure mercury burner to preserve
sensitive specimens from being burned out or
bleached out.
Switching the high-pressure mercury
burner on and off frequently decreases
its life and causes delays, as the lamp can only
be switched back on after it has cooled. Instead,
you can close the UV shutter and reopen it
when you return to your workplace.
Close the UV shutter when you do not want to
view the specimen with fluorescence illumina-
tion, but only with transmitted light.