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Leica M-series User Manual 116
FIM – Fluorescence Intensity Manager
FIM consists of a diaphragm disk with pinhole
diaphragms of varying transmission capaci-
ties. The intensity of the excitation light can
be reduced in five fixed steps. The light can
be changed from 100% to 55%, 30% and 17%
down to 10%.
The advantage of this procedure is its absolute
reproducibility and fast switching between the
intensity levels. A separate FIM level can be
stored for every fluorescence filter. Different
intensities of fluorescence can thus be cali-
brated to one another.