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10. Trouble Shooting
Problem
Polarization
No polarization contrast is possible.
Fluorescence
The image is completely dark (no fluorescence).
The fluorescence is too weak.
Display
The display flashes.
FAIL! appears.
Cause/Remedy
Bring the polarizer and analyzer into cross po-
sition until they reach maximum darkness
(without specimen) (→
p. 55, 59).
Open the shutter (→
p. 57).
Select the incident light axis (IL) (→
p. 36).
Check the antigen-antibody combination.
Insert the Booster Lens (→
p. 29).
Center the lamp (→
p. 41ff).
Insert a new lamp (→
p. 20f).
Rotate an appropriate objective for the con-
trast method into the light path.
Check insertion of objectives, cubes, etc.