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8. Imaging Procedure for Leica DM4000 B/DM5000 B
8.2 Fluorescence
• Switch to the fluorescent light axis (FLUO) by
pushing the TL/IL button.
• Insert a specimen and rotate a suitable objec-
tive into place.
• The current fluorescence cube is indicated on
the display.
• Closing the incident light shutter protects your
specimen from fading.
Do so by pressing the SHUTTER variable key.
(For key occupation please see “Identification
Sheet”.)
The display indicates the symbol: ↓
• Selecting the fluorescence filter cube:
Press the variable keys
Cube or Cube
• The fluorescence intensity can be increased
using the Booster Lens on the right side of the
stand (Fig. 69).
• For multifluorescence, use of a Excitation
Manager is recommended. The Excitation
Manager is inserted into the right side of the
stand up to the last stop (Fig. 70).
• Using Booster Lens
and Excitation Manager,
the Excitation Manager can be inserted into
the back receptacle.
Fig. 70 Inserting the Excitation ManagerFig. 69 Inserting the Booster Lens
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