Leica DM4000B Microscope & Magnifier User Manual


 
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9. Imaging Procedure for Leica DM4000 M
9.1 Incident Light
9.1.1 Bright Field
Switch to the incident light axis (IL) by pushing
the TL/IL button.
Select the BF (bright field) contrast method.
Do so by pressing the BF variable key.
Alternatively: Press the CHANGE RL
variable key.
(For key occupation please see “Identification
Sheet”.)
The display indicates BF.
Insert a specimen.
Rotate an appropriate objective into place.
Bring the image into focus using the focus dial
and set the brightness using the INT function
key.
9. Imaging procedure
for Leica DM4000 M
9.1.2 Dark Field
Switch to the incident light axis (IL) by pushing
the TL/IL button.
Select the DF (dark field) contrast method.
Do so by pressing the DF variable key.
Alternatively: Press the CHANGE RL
variable key.
(For key occupation please see “Identification
Sheet”.)
The display indicates DF.
The DF reflector is turned into the beam
path.
Insert a specimen.
Rotate an appropriate objective into place.
Bring the image into focus using the focus dial
and set the brightness using the INT function
key.
Notes:
The maximum objective aperture which may
be used for dark field is 0.75. All objectives
with greater aperture are automatically
blocked for this procedure ("DF" flashes in the
display).
When selecting the dark field method, the
aperture diaphragm is opened completely and
may not be adjusted. To avoid errors in opera-
tion, the function keys for setting the aperture
diaphragm (AP) are locked.
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