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9. Imaging Procedure for Leica DM4000 M
9.1.4 Interference Contrast
• Switch to the incident light axis (IL) by pushing
the TL/IL button.
• Insert a specimen and rotate a suitable objec-
tive into place.
• Select the DIC contrast method.
Do so by pressing the DIC variable key.
Alternatively: Press the CHANGE RL
variable key.
(For key occupation please see “Identification
Sheet”.)
The display indicates ICR.
• The ICR filter cube (containing polarizer and
analyzer) is automatically brought into the light
path on the incident light axis. Insert the objec-
tive prism slide into the prism slot (71.2).
Alternatively:
• Rotate the ICR polarizer (71.3) and the IC/P
analyzer (72.1) on the stand manually into the
light path.
• Insert the objective prism slide into the prism
slot (71.2).
• For fine adjustment, rotate the knurled screw
(71.1) on the objective prism slide.
9.2 Transmitted Light
9.2.1 Bright Field
• Switch to the transmitted light axis by pushing
the TL/IL button.
• Select the BF (bright field) contrast method.
Do so by pressing the BF variable key.
Alternatively: Press the CHANGE RL
variable key.
(For key occupation please see “Identification
Sheet”.)
The display indicates BF.
• Insert a transmitted light specimen.
• Rotate an appropriate objective into place.
• Use the focus dial to bring the image into fo-
cus and set the brightness using the INT func-
tion key.
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