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8. Imaging Procedure for Leica DM4000 B/DM5000 B
8.1 Transmitted Light
8.1.1 Bright Field (TL)
• Switch to the transmitted light axis (TL) by
pushing the TL/IL button.
• Select the BF (bright field) contrast method.
Do so by pressing the BF variable key.
Alternatively: Press the CHANGE TL
variable key.
(For key occupation please see “Identification
Sheet”.)
The display indicates BF.
• Insert a transmitted light specimen.
• Rotate an appropriate objective into place.
• Bring the image into focus using the focus dial
and set the brightness using the INT function
key.
8.1.2 Phase Contrast
• Switch to the transmitted light axis (TL) by
pushing the TL/IL button.
• Select the PH contrast (phase contrast) method.
Do so by pressing the PH variable key.
Alternatively: Press the CHANGE TL
variable key.
(For key occupation please see “Identification
Sheet”.)
The display indicates PH.
• Insert a transmitted light specimen.
• Rotate an appropriate objective into place.
Objectives that are suitable for phase contrast
are engraved with PH.
• Bring the image into focus using the focus dial
and set the brightness using the INT function
key.
8. Imaging Procedure
for Leica DM4000 B/ Leica DM5000 B
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