Leica RM2245 Microscope & Magnifier User Manual


 
61
Leica RM2245
7. Customized solutions
1. Thick/thin sections
The sections alternate between
being thick and thin. In extreme
cases, there are no sections
whatsoever.
Check the clamping positions
and repeat the clamping
process
Laterally displace the knife
holder or insert a new blade.
Insert a new pressure plate or
use a new knife holder.
Methodically experiment with
larger clearance angle settings
until you have found the opti-
mum angle.
Use another area of the blade or
a new blade.
Cool the specimen before
sectioning.
Reduce the sectioning speed.
Remove paraffin from this area
on a regular basis.
Select a slower sectioning
speed.
Methodically reduce the clear-
ance angle setting until you
have found the optimum angle.
Check all screw and clamp
connections on the specimen
holder system and the knife
holder. If necessary, tighten the
levers and screws.
Adjust the sectioning speed
and/or section thickness when
sectioning. Select a thinner
section.
Problem Corrective actionPossible cause
Insufficient clamping of blade,
knife holder, specimen or
orientation.
The blade is dull.
The pressure plate is damaged
or incorrectly adjusted.
Clearance angle of the knife/
blade too small.
The blade is dull.
The specimen is too warm.
The sectioning speed is too fast.
There is an accumulation of
paraffin on the rear pressure
plate of the knife holder.
The sectioning speed is too fast.
The clearance angle is too wide.
Insufficient clamping of speci-
men clamp and/or knife holder.
Too great of a sectioning force
was applied.
3. "Stripes" in sections
For knife holder E
2. Section compression
The sections are very com-
pressed, show folds or are
squeezed together.
4. Noises during sectioning
The knife "sings" when
sectioning hard specimens.
The sections show scratches or
chatter marks.
7.2 Possible faults
5. High blade consumption