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Contents
6. Startup ........................................................ 31
6.1 Functional Principle.................................. 31
6.2 Switching on the Microscope ................ 34
6.3 The Display
(Leica DM4000 B/DM4000 M) ................. 35
6.4 The Function Keys .................................... 36
6.5 Köhler Illumination.................................... 37
6.6. Checking Phase Contrast Rings............. 39
6.7 Adjusting the Light Sources.................... 40
7. Operation ................................................... 46
7.1 Switching on the Microscope ................ 46
7.2 Stages and Specimen Displacement .... 46
7.3 Focusing ..................................................... 47
7.4 Tubes...........................................................
48
7.5 Eyepieces ................................................... 49
7.6 Objectives .................................................. 50
7.7 Magnification Changer ............................ 51
7.8 Light Sources............................................. 52
7.9 Aperture Diaphragm and
Field Diaphragm ........................................ 52
8. Imaging Procedure for
Leica DM4000 B/Leica DM5000 B ......... 53
8.1 Transmitted Light ...................................... 53
8.1.1 Bright Field ...................................... 53
8.1.2 Phase Contrast ............................... 53
8.1.3 Dark Field......................................... 54
8.1.4 Polarization ..................................... 55
8.1.5 Differential
Interference Contrast.................... 56
Contents
1. Important Notes about this Manual ..... 7
2. Safety Notes .............................................. 8
2.1. General Safety Notes ............................... 8
2.2. Electrical Safety ........................................ 8
3. Overview of the Instrument .................... 10
4. Unpacking the Microscope .................... 14
5. Assembling the Microscope .................. 16
5.1 Stage ........................................................... 17
5.2 Condenser .................................................. 18
5.3 Tube and Eyepieces ................................. 19
5.4 Objectives .................................................. 19
5.5 Light Sources for the
Transmitted Light Axis ............................. 20
5.6 Light Sources for the
Incident Light Axis .................................... 21
5.7 Equipping the
Incident Light filter turret ........................ 26
5.8 Polarizer and Analyzer ............................. 27
5.9 DIC Prisms.................................................. 28
5.10 Optional Accessories ............................... 29
5.11 Connection to the Power Supply............ 30
5.12 Connection to the
CTR5000 Electronics Box ......................... 30