Leica DM4000M Microscope & Magnifier User Manual


 
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6. Startup
Open the field diaphragm just enough for it to
disappear from the field of view (41d).
Caution:
Do not adjust the aperture diaphragm. The aper-
ture diaphragm is already set optimally for each
objective.
6.6. Checking Phase Contrast Rings
A
B
CD
Fig. 41 Köhler Illumination
a Field diaphragm not focused, not centered
b Field diaphragm focused, but not centered
c Field diaphragm focused and centered
Diameter is too small, however
d Field diameter (light) = Field diameter (view)
(Köhler Illumination)
Fig. 40 Condenser centering
1 Centering bolts
11