Leica DM4000M Microscope & Magnifier User Manual


 
37
6. Startup
However, depending on how the condenser is
disassembled and reassembled, it may be nec-
essary to re-adjust the condenser in some
cases. Therefore, check the condenser
centering.
The following procedure is provided for the
transmitted light-bright field illumination.
Select an objective with moderate
magnification (10x-40x).
Activate the transmitted light axis by pushing
the TL/IL button (38.1). "TL" appears in the first
line of the display.
Choose "bright field" as the contrast method by
pressing the BF (one of the variable function
keys, behind the focus dials).
"TL BF" appears in the first line of the display.
Insert the specimen in the stage’s specimen
holder (39.3).
Focus on the specimen. The focus wheel on
the left side of the stage allows focus adjust-
ment in large and small increments. On the
right side of the stage, there is also a focus
wheel for fine focus adjustment.
Set the light intensity using the INT keys (38.3).
Close the field diaphragm with the FD function
key (38.2) until the edge of the diaphragm ap-
pears in the specimen plane.
Using the condenser height adjuster (39.4), ad-
just the condenser until the edge of the field
diaphragm appears in sharp relief.
If the image does not appear in the middle of
the field of view (41c), the condenser must be
moved into the middle of the field of view with
the help of the two leveling screws (40.1).
2
1
3
Fig. 39 Stage with specimen holder
1 Object motion (X direction)
2 Object motion (Y direction)
3 Specimen holder
4 Condenser height adjuster
4