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Expandability to Support Diverse Application.
Application Standard Functions Optional Functions
Molecular interaction and Intracellular diffusion measurement
molecular concentration Calculation of diffusion coefficients for intracellular molecules, and analysis of
analysis
—
molecular binding and changes in molecular density.
Supports a wide range of methods (RICS/ccRICS, point FCS/point FCCS and
FRAP).
Software Required: Diffusion measurement package
Laser Light stimulation Acquires images while rapidly switching SIM scanner system
the built-in laser between imaging and Performs simultaneous imaging and laser light stimulation. Provides detailed
laser light stimulation. settings for laser light stimulation including position and timing.
Features tornado scanning for high- Features tornado scanning for high-efficiency bleaching using laser light
efficiency bleaching using laser light stimulation.
stimulation. Equipment Required: SIM scanner, laser combiner (dual fiber version)
Multi-point laser light stimulation system
Register multiple points for laser light stimulation, and program the respective
stimulation order, stimulation time and type of stimulation (continuous laser light
or pulse laser light).
Software Required: Multi-stimulation software
Multi-dimensional Long time-lapse system
time-lapse imaging Microscopes equipped with zero drift compensation (ZDC) acquire each image at
a set focus plane. The microscope CO2 incubator maintains cell activity for a long
period for continuous imaging.
— Equipment Required: IX81-ZDC microscope, CO2 incubator
Multi-point scanning system
Register multiple points for repeated image acquisition. Efficiently observe multiple
cells in parallel on 35-mm dishes, microplates or chamber slides.
Software and Equipment Required:
Multi-area time-lapse software, motorized XY stage**
3D mosaic imaging 3D mosaic imaging system
Continuous imaging of adjacent fields of view and mosaic imaging to form a
— composite image.
Acquisition of adjacent Z-series images for 3D mosaic imaging.
Software and Equipment Required:
Multipoint time-lapse software, motorized XY stage**
TIRFM TIRFM imaging
Uses the laser from the laser combiner to provide evanescent illumination, for
—
imaging the movement of molecules near the glass surface, such as cell
membranes and adhesion factors.
Software and Equipment Required: TIRFM unit*, TIRF objective, high-
sensitivity CCD camera**, CCD camera control software**
FRET Provides FRET analysis functions. CFP-YFP FRET
Diode laser offers exceptional stability Ratio imaging and sensitized emission.
and long life. Available 440 nm diode laser is optimized for CFP-YFP FRET experiments
Supports FRET efficiency methods.
measurements using acceptor Diode laser offers exceptional stability and long life.
photobleach method. Equipment Required: LD 440 nm Laser
Multi-color imaging Three-channel detector for Imaging blue dyes
simultaneous acquisition of Available 405-nm laser for image acquisition of multi-stained samples labeled with
fluorescence images from three V-excitation fluorescent dyes such as DAPI, Hoechst and Alexa 405.
different dyes. Equipment Required: LD 405 nm laser
Sequential mode for acquisition of
Simultaneous four-color imaging
fluorescence images without cross-talk.
Fourth channel detector can be easily added to simultaneously acquire images of
Fluorescence can also be separated
four colors.
using unmixing (only available on
Equipment Required: 4-channel detector
spectral scan unit).
Colocalization analysis Easily determine if labeled substances High-accuracy colocalization analysis
are present locally in the same New 60x oil-immersion objective offers image acquisition with exceptional
locations. positional accuracy coefficient.
Calculate of Pearson coefficients, Equipment Required: PLAPON 60xOSC
overlap coefficients and colocalization
indices.
* SIM scanner and TIRFM scanner cannot be installed on the same system.
** For more information about peripheral equipment, contact your Olympus dealer.
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