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Application/ Molecular Interaction Analysis
3D Mosaic Imaging
Mosaic imaging is performed using a high-magnification objective to acquire continuous 3D (XYZ) images
of adjacent fields of view using the motorized stage, utilizing proprietary software to assemble the
images. The entire process from image acquisition to tiling can be fully automated.
Automated from 3D Image Acquisition to Mosaic Imaging
Multi-area time-lapse software automates the process
from 3D image acquisition (using the motorized XY
stage) to stitching. The software can be used to easily
register wide areas, and the thumbnail display
provides a view of the entire image acquired during
the mosaic imaging process.
Coordinate Information
Thumbnail
CNS markers in normal mice
Objective : PLAPON60x
Zoom : 2x
Image acquisition numbers (XY): 32 x 38, 48 slices for each image
Courtesy of: Dr. Mark Ellisman PhD, Hiroyuki Hakozaki, MS Mark Ellisman
National Center for Microscopy and Imaging Research (NCMIR),
University of California, San Diego
Mosaic Imaging for 3D XYZ Construction
Composite images are quickly and easily prepared using the stitching function, to form an image over a wide area. 3D construction can
also be performed by acquiring images in the X, Y and Z directions. Tiled images can be enlarged in sections without losing resolution.
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