Olympus FV1000 Microscope & Magnifier User Manual


 
Application
15,000 20,000 30,00025,000
Time (ms)
35,000 40,000
200
400
600
800
1,000
1,200
1,400
1,600
1,800
2,000
2,200
2,400
Intensity
CH1
CH2
CH1
CH2
Measurement
Diffusion measurement and molecular interaction
analysis.
Light Stimulation
FRAP/FLIP/Photoactivation/Photoconversion/Uncaging.
Multi-Dimensional Time-Lapse
Long-term and multiple point.
3D Mosaic Imaging
High resolution images stitched to cover a large area.
Multi-Color Imaging
Full range of laser wavelengths for imaging of diverse
fluorescent dyes and proteins.
3D/4D Volume Rendering
One-click 3D/4D image construction from acquired
XYZ/T images.
Change the angle of 3D image with a single click.
Colocalization
Configurable threshold values for fluorescence
intensities on the scatterplot.
Accurate colocalization statistics and visualization of
colocalized area on image.
FRET
Configuration wizard simplifies the setting of FRET
experimental procedures.
Optimal laser excitation wavelengths for CFP/YFP
FRET.
Image of variations in calcium concentration of HeLa cells
expressing YC3.60 when stimulated with histamine.
Reference:
Takeharu Nagai, Shuichi Yamada, Takashi Tominaga, Michinori
Ichikawa, and Atsushi Miyawaki 10554-10559, PNAS, July 20,
2004, vol. 101, no.29
14