Olympus FV1000 Microscope & Magnifier User Manual


 
Low Chromatic Aberration Objective
Best Reliability for Colocalization Analysis
A new high NA oil-immersion objective minimizes chromatic
aberration in the 405–650 nm region for enhanced imaging
performance and image resolution at 405 nm. Delivers a high
degree of correction for both lateral and axial chromatic
aberration, for acquisition of 2D and 3D images with excellent
and reliable accuracy, and improved colocalization analysis. The
objective also compensates for chromatic aberration in the near
infrared up to 850 nm.
New Objective with Low Chromatic Aberration Delivers
World-Leading Imaging Performance.
NEW
NEW
Low Chromatic PLAPON60xOSC
Aberration Objective Magnification: 60x
NA: 1.4 (oil immersion)
W.D.: 0.12 mm
Chromatic aberration compensation range: 405–650 nm
Optical data provided for each objective.
3D image
Tubulin in Ptk2 cells labeled with
two colors (405 nm, 635 nm) and
compared.
Improved Flatness and Resolution at 405 nm
Better flatness reduces the number of images for tiling.
450400
-0.5
0.0
Focal plane (µm)
0.5
Lateral Chromatic Aberration
Wavelen
g
th (nm)
500 550 600
650
PLAPON 60xOSC
UPLSAPO60xO
UPLSAPO60xO
Chromatic Aberration Comparison for PLAPON 60xOSC and
UPLSAPO 60xO
Performance Comparison of PLAPON 60xOSC and UPLSAPO 60xO
PLAPON60xOSC
Axial chromatic
aberration (Z direction)
Compared for PSF fluorescent
beads (405 nm, 633 nm).
Lateral chromatic
aberration (X-Y direction)
Compared for PSF fluorescent
beads (405 nm, 488 nm, 633
nm).
*Chromatic aberration values are design values and are not guaranteed values.
Lateral and Axial Chromatic Aberration
Small Degree of
Chromatic Aberration
Large Degree of
Chromatic Aberration
Axial chromatic
aberration
(Z direction).
Lateral chromatic
aberration (X-Y
direction at FN6).
Approx.
0.5 µm
Approx.
0 µm
Approx. 0.1 µm Approx. 0.2 µm
Objective
PLAPON60xOSC UPLSAPO60xO
Flatness Comparison Image at 1x Zoom
9