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Description
Fluorescence technique
Some substances fluoresce when irradiated with short-wave
light. This property is utilized in the fluorescence technique,
in which certain structures and features which do not fluo-
rescence can be tagged with a fluorescing dye. An example
is green-fluorescing protein (GFP), which is used in molecular
biology.
Stereo-fluorescence
With Leica fluorescence systems you can complete unprepared
fluorescing specimens to be non-destructively studied in three
dimensions, mani pulated, sorted and recorded. The intense light
emitted by the mercury-vapour burner, used in conjunction with
selected filter sets, enables even the finest structures to be
differentiated and expands the amount of information revealed
by the incident-light fluorescence technique.
Fluorescence stereomicroscope
The Leica MZ10 F with 10:1 zoom is the routine stereo-
microscope for fluorescence applications. The patented sepa-
rate beam path (TriBeam™) for the fluorescence illuminator and
the patented filter system (FLUOIII™) together produce excellent
fluorescence images.
Stereo-fluorescence module
The stereo-fluorescence modul can be combined with the
Leica stereomicroscope models MS5, MZ6, MZ7.5, MZ9.5,
MZ12.5, MZ16, and with older models.
User manual
The present user manual describes only the functions of the
fluorescence illuminator, the use of the filter sets, and the fitting
of the fluorescence module. For detailed information about the
use of the stereomicroscope, its care and its safety, please refer
to those sections of the separate manual M2-105-0EN which
relate to the Leica MZ12.5 with 12.5:1 zoom.
Safety concept
Before you set up the instrument and before you fit the
fluorescence illuminator, read:
• thisusermanual,observingthenotesrelatingtosafety
• theusermanualforyourstereomicroscope,observingthe
notes relating to safety and care.
Permitted uses
Leica stereo-fluorescence systems are equipped with a special
fluorescence illuminator. They are intended for the three-
dimensional observation of fluorescing objects. They consist of:
– a stereomicroscope with stand, binocular tube and
eyepieces
– an integrated FLUOIII filter system (for MZ10 F)
or a separate stereo-fluorescence module
– appropriate filter sets with barrier- and excitation filters,
a light stop, and individually-selectable filters
– a UV protection screen
– a lamp housing with high-pressure mercury vapour burner
– a supply unit with power cable.
Prohibited uses
The use of Leica stereo-fluorescence systems in a different
manner from that described in this user manual can lead to inju-
ry, malfunction and damage.
– Do not fit different plugs or cables
– Do not dismantle or modify components unless instructions
for doing so are given in the user manual
– Components may only be opened by authorized personnel.
Place of use
Leica stereo-fluorescence systems are intended for use in
closed rooms and may not be used outdoors.
Responsibilities of person in charge of instrument
– Ensure that the Leica stereo-fluorescence systems are
operated, maintained and repaired only by authorized and
trained personnel
– Ensure that personnel who use the instrument have read
and understood this user manual and in particular all safety
instructions.