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Leica DM750 P Manual 31
Koehler Set up
1. Close the Koehler field diaphragm on the base of the microscope so
that its iris leaves are present within the field of view when looking
into the eyepieces.
2. Use the condenser focus knob on the left-hand side of the substage to
bring the leaves of the field diaphragm into sharp focus.
3. Turn the condenser centering thumbscrews simultaneously to center
the image of the field diaphragm in the field of view.
4. Open the field diaphragm until the iris leaves disappear just beyond
the field of view.
Koehler Set up