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Figure 9. Light pointer controls
2.8 Koehler illumination
Koehler illumination needs to be adjusted in the beginning of every imaging session
for optimal illumination. Proceed as follows:
Instruction
View in eyepiece
1. Use 10x objective and
front lens. Focus on the
sample.
2. Close the luminous-field
diaphragm by turning the
adjustment wheel clockwise.
3. Move the condenser up or
down so that the diaphragm
edges are in focus. The
microscope is designed so
that in the imaging path, field
diaphragm should be in
conjugate plane with the
specimen plane (in focus at
the same time). You adjust
this by adjusting the height of
the condenser.