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1
GENERAL USER INFORMATION ..............................................................................1
2
SETTINGS FOR TRANSMITTED LIGHT MICROSCOPY...................................... 1
2.1
Turning on the microscope....................................................................................................1
2.2
XY-stage................................................................................................................................1
2.3
Placing the sample.................................................................................................................2
2.4
Objectives..............................................................................................................................2
2.5
Focusing ................................................................................................................................3
2.6
Eye pieces (oculars)...............................................................................................................4
2.7
Dual viewing attachment.......................................................................................................4
2.8
Koehler illumination..............................................................................................................6
2.9
Polarization contrast..............................................................................................................8
2.10
Filters.....................................................................................................................................8
2.11
Dark-field microscopy...........................................................................................................9
2.12
Turning off the microscope ...................................................................................................9
3
IMAGE ACQUISITION ................................................................................................10
3.1
Accessing the computer.......................................................................................................10
3.2
View Finder application ......................................................................................................10
3.3
Adjustments before image acquisition.................................................................................10
3.3.1
Capture resolution...........................................................................................................10
3.3.2
Sensitivity .......................................................................................................................10
3.3.3
Exposure Mode...............................................................................................................11
3.3.4
White balance .................................................................................................................11
3.3.5
Levels adjustment...........................................................................................................11
3.3.6
Focus tool .......................................................................................................................12
3.3.7
Over/Underexposure.......................................................................................................12
3.3.8
Averaging .......................................................................................................................12
3.4
Capturing the image ............................................................................................................13
3.5
Viewing images...................................................................................................................13
3.6
Post-imaging adjustments....................................................................................................13
3.6.1
Cropping the image.........................................................................................................13
3.6.2
Scale bars........................................................................................................................14
4
SAVING AND TRANSFERRING IMAGES ...............................................................14
5
FINISHING YOUR IMAGING.....................................................................................14
6
TROUBLESHOOTING.................................................................................................15
6.1
There is no light...................................................................................................................15
6.2
Light is too dark ..................................................................................................................15
6.3
Problems with focus or picture clarity.................................................................................15
6.4
View is partial .....................................................................................................................15
6.5
Colors have red/yellow tint .................................................................................................15
6.6
Ocular view is not at the same focus as the camera view....................................................15
6.7
Computer login fails............................................................................................................16