Philips CM12 Microscope & Magnifier User Manual


 
Erwin Sabio Philips CM12 Alignment & Setup Manual Page 12
Sample Image Adjustment-Optimizing Eucentric (Z) Height
Figure 14 The center of the four holes
in this sample can be chosen as a
reference point for z-height adjustment.
1.
Adjust the beam intensity on the sample or increase the
magnification by turning the magnification knob [14] to
see certain structures of the sample. Then pick a
feature on the center of the sample as a reference point
for adjusting the Z-height.
2.
Unlock the tilt lever and tilt the sample from +10 degrees
to -10 degrees to see how far the reference point
moves. Return the sample to 0 tilt.
3.
Turn the z-height knob [27] to one direction then tilt the
sample +/-10 degrees again to check the movement of
the reference point. If the movement becomes smaller,
continue turning the knob in that direction. Otherwise,
turn the other way.
4.
Continue alternating between turning the z-knob and
tilting the device until the movement of the reference
point reaches a minimum. At this point, the optimal z-
height has been reached.
5.
Return the tilt to 0 degree then put the lever in lock
position.
Sample Image Adjustment-Adjusting Rotation Center
1.
As in z-height adjustment, begin by choosing a central
feature or the sample as a reference point.
2.
Press the ALGN button on the control panel and select
ROT CENTER from the onscreen menu.
3.
The image should wobble in and out i.e. symmetrically
around the center when the objective rotation center is
aligned. If the reference point moves in addition to the
wobbling, adjust the multifunction X/Y knobs until the
reference point stays put.
4.
Another way to check if the rotation center is aligned is
to got to different focus steps by turning the outer knob
of the focus-focus step knob [20]. The wobbling of the
sample should appear relatively the same at different
focus steps.