Filter
Top Products
English
Page 22 of 24
English
Leica Biosystems Leica HER2 FISH System - 30 Test Instructions for Use TA9217 EN-CE-Rev_D 08/04/2013
Problem Probable Cause Remedial Action
Nonspecic
background
uorescent
signal/staining
Inadequate post hybridization
washing
Increase post hybridization wash incubation
time.
Inappropriate bulk reagents
dispensed
Ensure all Leica BOND reagents have been
allocated into appropriate bulk containers
and placed into appropriate positions on the
instrument.
Inadequate deparanization
of slides
Ensure Dewax is selected in the Preparation
eld of the Add slide dialog.
Nonspecic cross-reaction
with areas of tissue necrosis
Ensure a formalin-based xative is used
and that processing schedules are suitable
for the specimen undergoing testing. If
possible, retest case using another block.
If this is not possible, assess in conjunction
with a corresponding H&E stained section,
and select areas which show best xation
patterns.
Sections adhered to slides
using alternative adhesives
Use Leica BOND Plus Slides (S21.2113).
Poor preservation
of tissue
morphology
Inadequate tissue xation and
processing
Ensure a formalin-based xative is used
and that processing schedules are suitable
for the specimen undergoing testing. If
possible, retest case using another block.
If this is not possible, assess in conjunction
with a corresponding H&E stained section,
and select areas which show best xation
patterns.
Inappropriate pretreatment Adjust pretreatment protocol (HIER or
Enzymatic Digestion).
Tissue detached
from patient/
control slide(s)
Use of incorrect type of slides
or inadequate draining of
section
Ensure appropriate slides are used for patient/
control sections (e.g. Leica BOND Plus Slides
S21.2113). Ensure slides receive adequate
draining and are incubated for 1 hour at 60 °C.
Table 6. Leica HER2 FISH System - 30 Test Troubleshooting Guide.
If any problems associated with the Leica HER2 FISH System - 30 Test fall outside the scope
of the troubleshooting guide please contact your local Leica Biosystems Technical Services
Department or Distributor for assistance.