Olympus FV300 Microscope & Magnifier User Manual


 
Preset the conditions for image
acquisition and loading
Storage of Acquisition Settings enables
immediate, one-touch recall of all the
relevant experimental settings and
conditions. Adding new conditions or
altering existing ones is quick and easy.
AOTF: flexible control of the laser
intensity to meet the specific
demands (optional)
The laser exposure will be limited within
the scanning area by default, minimizing
unnecessary bleaching of the specimen.
Option includes:
•Any laser intensity for any excitation area
("Region of Excitation" mode)
•Multiple laser applications
•AOTF controller that provides easy link
with external equipment
ZoomIn scanning
Zoom scanning can be
conducted over any
designated rectangular area.
Since only the region of the
targeted, zoomed-in area is acquired,
scan time and laser exposure of the
specimen is minimized.
Point scanning
The ultimate in fast scanning,
the point scan enables
accurate quantitation of
intensity changes during rapid
physiological events.
Line scanning
A single line may be scanned,
oriented at any angle in the XY
plane. This fast scanning
option permits accurate
quantitation of physiological events such
as Calcium waves or sparks.
Free line scanning
Intensity changes may be
measured over a given period
of time along the length of a
freely drawn line, such as the
trace of an axon or along a cellular
junction.
Clip scanning
By cropping the image, selected areas
can be cut out of complex image stacks.
3
Flexible setting of scanning size,
zoom, movement and rotation
The observation field and scanning area
are both displayed graphically. Settings
can be confirmed while scrolling through
the zoom ratios. The “pan” button lets the
operator move the image acquisition area
at will, and rotation scanning of images is
also possible.
Simplified toolbar
A newly designed toolbar with various
dedicated buttons has greatly improved
ease of use. The user can execute a
succession of selected processes with
one-click operation for each.
Ultimate ease of operation and monitor display.
Software Graphical User Interface
Dye selection display
When a fluorescence dye is chosen, the
laser and light path settings are selected
automatically, with each of the selected
fluorescence dyes displayed graphically
on the monitor.
Innovative scanning method for
improved performance
ZoomIn
Spot
SlantLine
FreeLine
20%
50%
B
Versatile display options
Exchange between condensed and full
image display modes can be performed
with a single touch. Individual panel
layouts can be changed at will, and the
panel in use can be placed in any desired
position.