Leica DM100 Microscope & Magnifier User Manual


 
Leica DM100 User Manual 30
Oil Immersion Technique (only 100× objective)
Oil Immersion Technique
1. Search for the area on the specimen slide
that you want to study.
2. Move the specimen stage down to the
lowest position using the coarse adjust-
ment knob.
3. Add a drop of immersion oil from Leica
to the area of the specimen slide you are
studying.
4. Rotate the 100× objective to the working
position.
5. Slowly move the specimen stage upwards
using the coarse adjustment knob until
the oil drop on the slide comes into direct
contact with the lens of the oil immersion
objective.
6. Hold the knurled ring on the objective
nosepiece and swing the objective back
and forth to eliminate air bubbles. Then
bring the oil objective into the final posi-
tion, so that the oil drop is between the
front lens of the objective and the speci-
men slide.