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Coolpix 4500 Camera Settings for Microscopy
1. Turn the camera on and select the infinity mode by pressing the Manual Focus (MF) button until the icon
with two mountain peaks is displayed in the upper-right of the monitor display. Note that the flash turns
off automatically when focus is set to infinity See p.57
2. Hold down the Mode button and simultaneously turn the command dial to select A, Aperture-Priority Auto
mode. The camera will set shutter speed automatically for best results.
3. Rotate the command dial to set the F value to the lowest possible f-stop number. The approximate range
value (F2.5 - F4.0) is dependent on the zoom setting.
4. Use the zoom buttons (W-T) to fill the field of view. Use the LCD screen to determine best image. An
image without black cut-off corners or circular images is generally possible at mid-range of the zoom
setting.
Additional Settings in Menu Selection
Press Menu for the Shooting Menu (See p. 91) for camera settings available only in the P, S, A, and M mode
and only when the camera is in shooting mode. The following are recommended options for photomicrography:
? White Balance: The default setting is Auto. Select a white balance setting that matches the microscope
light source; incandescent for tungsten or halogen illumination. Select White Bal Preset for unusual
lighting conditions. See p. 92
? Metering: The default setting is Matrix. Setting it to Spot on a targeted sample with a very dark or a very
bright background would ensure most appropriate exposure. See p. 95
? Continuous: The default setting is S, single shutter release. Using other settings, continuous sequence
of pictures may be taken while the shutter-release button is held down. See p. 96
? Best Selector: Use default setting Off. See p.98
? Image Adjustment: The default setting is Auto. Certain illumination techniques, such as DIC, phase
contrast, OCC, etc., may require special brightness and control adjustments for best results. See p. 99
? Saturation Control: The Black&White setting will enhance black and white samples. See p. 99-100
? Image Quality: Image quality settings other than HI are compressed as they are saved into memory.
Compression selectively reduces the quality of an image. See p. 102
? Image Sharpening: The default setting is Auto. See p. 105
Nikon Instruments Inc.
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