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INSULIN-L-U
Page 2
Novocastra
™
Liquid Mouse Monoclonal Antibody
Insulin
Product Code: NCL-L-INSULIN
Intended Use
For in vitro diagnostic use.
NCL-L-INSULIN is intended for the qualitative identication by light microscopy of human insulin in parafn sections. The clinical
interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be
evaluated within the context of the patient’s clinical history and other diagnostic tests by a qualied pathologist.
NCL-L-INSULIN is recommended for the assessment of insulin protein expression in normal and neoplastic tissues.
Summary and Explanation
The rst immunohistoperoxidase technique was reported by Nakane and Pierce.
1
Since then many developments have occurred, leading
to increased sensitivity over earlier techniques. A recent development has been the use of polymeric labeling. This technology has
been applied to both primary antibodies
2
and detection systems. The Novolink
™
Polymer Detection Systems utilize a novel controlled
polymerization technology to prepare polymeric HRP-linker antibody conjugates. Therefore, the problem of non-specic staining that can
occur with Streptavidin/Biotin detection systems due to endogenous biotin does not occur.
Principle of Procedure
Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specic antibody
to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate
with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The
specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope and aid in the differential
diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.
Reagent
NCL-L-INSULIN is a liquid tissue culture supernatant containing sodium azide as a preservative.
Clone
2D11-H5
Immunogen
Insulin conjugated to bovine serum albumin carrier protein.
Specicity
Human insulin. Also reacts with swine and bovine insulin, but not mouse or rat insulin.
Ig Class
IgG1
Total Protein Concentration
Refer to vial label for lot specic total protein concentration.
Antibody Concentration
Greater than or equal to 8.5 mg/L as determined by ELISA. Refer to vial label for lot specic Ig concentration.
Warnings and Precautions
This reagent has been prepared from the supernatant of cell culture. As it is a biological product, reasonable care should be taken when
handling it.
This reagent contains sodium azide. A Material Safety Data Sheet is available upon request or available from
www.LeicaBiosystems.com
Consult federal, state or local regulations for disposal of any potentially toxic components.
Specimens, before and after xation, and all materials exposed to them, should be handled as if capable of transmitting infection and
disposed of with proper precautions.
3
Never pipette reagents by mouth and avoid contacting the skin and mucous membranes with
reagents and specimens. If reagents or specimens come in contact with sensitive areas, wash with copious amounts of water. Seek
medical advice.
Minimize microbial contamination of reagents or an increase in non-specic staining may occur.
Incubation times or temperatures, other than those specied, may give erroneous results. Any such changes must be validated by the
user.
Storage and Stability
Store at 2–8 °C. Do not freeze. Return to 2–8 °C immediately after use. Do not use after expiration date indicated on the vial label.
Storage conditions other than those specied above must be veried by the user.
The signs indicating contamination and/or instability of NCL-L-INSULIN are: turbidity of the solution, odor development, and presence of
precipitate.
Specimen Preparation
The recommended xative is 10% neutral-buffered formalin for parafn-embedded tissue sections.
Total Protein